Sequestering agents like ethylenediamine tetraacetate (EDTA) are used to prevent the formation and deposition of Ca and Mg soaps and to clarify formulations when hard water is used for the production of shampoo and bath preparations. As a positive side-effect they can also give support to the preservation system.
The outer membrane of Gram-negative organisms like Pseudomonas is very complex and is stabilized by Ca and Mg ions. Strong chelating agents will destabilize this membrane making it more permeable for the preserving agents.
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