High Pressure Liquid CHROMATOGRAPHY

High Pressure Liquid CHROMATOGRAPHY or HPLC.
was the separation method where the still phase was placed in the column.
The tip of the other column was connected in the exerting pressure eluen
temperature.
The implement:
- Chromatography consisted of the system to channel the phase gram.
- the Place to put the substance that will be checked.
- the chromatography Column.
- the detection system  and the registration

The procedure:
- the Phase gram was pumped with the pressure from one or more reservoir in the column with the rate of the constant current.
- the Composition and the rate of the current from phasegram was stated in chromatography.
- the Solution to the test was put in the door to the injection (in being wrong one from three roads) :
a.Injection the valve was with the certain volume put used the hole injector.
B.Injection the varying volume valve was put used the injection valve.
C.Injection on coulomb where the varying volume was put used the injection implement through the partition.

Gas CHROMATOGRAPHY

GAS CHROMATOGRAPHY
Tool :
- the Source gas
- the Place of the sample injecting
- the chromatography Column
- the detection System and registration
- the still Phase that have the shape of solid or the liquid film was distributed level to the wall of the column.
The procedure: (tool was sophisticated, we Only injected).
- the Sample was injected directly to the column.
- the bearer's Gas flowed through the column in the speed or the constant pressure and afterwards through the detector that gave the response to the existence of sample components (took the form of the peaks of the graph).

Thin Layer Chromatography

Be the same as paper chromatography.
Their Absorbance from the thin layer uniform, soft dust.
The substance that was stuck on the sheet or the slab of glasses, plastic chromatography or metal (that often was straight of glasses 20x20 cm). the slab was dried by being baked first.
The procedure:
- washed the slab well used chromat acid .
- Rinse with water, dry.
- the Slab was covered with absorben.
- dry in an oven
- Let cold until the temperature of the room
- Just was used

paper CHROMATOGRAPHY

paper CHROMATOGRAPHY as absorben was the sheet of paper with the texture and the appropriate thickness. The separation was carried out through the work of the liquid phase. With absorbsi from paper.
Method :
a. Down chromatography
tool :

• The impermeable vessel vapour or chamber for the storage of solvent.
• Filter paper in a wide manner minimal 2,5 cm approximately almost be the same as high the vessel.

The method of the work procedure:
- the Substance that must be analyzed, was dissolved in appropriate solvent.
- the exact Volume the solution that has been still being measured in a micro pipette. Usually contain 1-20 µg. 
- the test substance was patched became spotty the measurement 6-10 mm with the distance not less than 3 cm.
- paper chromatography  was afterwards hung in the vessel by using the stick anti of siphon or clips held the tip of paper.
(beforehand was left first alone for a moment, to bored, in order to rise slowly or really was separated).
- was made an effort to get by paper that hanged down did not touch shelves or the wall of the vessel. The vessel was closed impermeable for saturation.

B.increase chromatography
the implement and the material were the same as descended chromatography , but the method : from top to bottom.