High Pressure Liquid CHROMATOGRAPHY

High Pressure Liquid CHROMATOGRAPHY or HPLC.
was the separation method where the still phase was placed in the column.
The tip of the other column was connected in the exerting pressure eluen
temperature.
The implement:
- Chromatography consisted of the system to channel the phase gram.
- the Place to put the substance that will be checked.
- the chromatography Column.
- the detection system  and the registration

The procedure:
- the Phase gram was pumped with the pressure from one or more reservoir in the column with the rate of the constant current.
- the Composition and the rate of the current from phasegram was stated in chromatography.
- the Solution to the test was put in the door to the injection (in being wrong one from three roads) :
a.Injection the valve was with the certain volume put used the hole injector.
B.Injection the varying volume valve was put used the injection valve.
C.Injection on coulomb where the varying volume was put used the injection implement through the partition.