Wednesday, May 21, 2008

column chromatography

Simple device took the form of the column, consisted of the tube and the stick to cram a lump of wool or cotton in the foundation of the tube and to press the absorber or absorben took the form of the porridge in the certain situation, the slab of porous glasses was placed in the foundation of the tube to support the contents of the tube.
The flow tube with the diameter was smaller was connected by means of merged or was connected with leak-proof continuation on the end the lower tube.
Absorben as the dry solid or as the porridge dipack in the tube of glasses chromatography or kuansa.
The solution and the substance were in a little solvent added at the top of the column and were allowed to flow through absorben.

Chromatography kinds

Chromatography kinds were:
  • Column Chromatography
  • Paper Chromatography
  • Thin Layer Chromatography
  • Gas Chromatography
  • the high achievement liquid Chromatography

separated and result chromatography


Separated is the mechanism that predominan in gas chromatography, the liquid of paper chromatography, Thin Layer Chromatography.
Results were checked by means of :
- could be seen direct
- was seen was supervised Violet's Ultra rays after was reactioned with the certain reagent.
- used the implement geigger muller if containing the radioactive substance.

Protracted substances

Protracted substances experienced:
- the solid phase
- the idle phase or chromatography (worked through absorbtion), as in the case of in absorbance or active aluminium chromatogram, silica gel (resin or the exchange of the ion).
- the Phase moved, eluen or the liquid (moved protracted substances through the medium up to was separated from the other protracted substance).

chromatography

Chromatography was the procedure where protracted substances were separated with the process of the differential migration dynamic in a system that consisted of two phases or more.
One of them moved continuously to the side of that was determined and each showing the mobility that was different was caused because of the difference and observation of the partition of the solubility of the mobility of the pressure of measurement vapour or propagation ion cargo.