paper CHROMATOGRAPHY

paper CHROMATOGRAPHY as absorben was the sheet of paper with the texture and the appropriate thickness. The separation was carried out through the work of the liquid phase. With absorbsi from paper.
Method :
a. Down chromatography
tool :

• The impermeable vessel vapour or chamber for the storage of solvent.
• Filter paper in a wide manner minimal 2,5 cm approximately almost be the same as high the vessel.

The method of the work procedure:
- the Substance that must be analyzed, was dissolved in appropriate solvent.
- the exact Volume the solution that has been still being measured in a micro pipette. Usually contain 1-20 µg. 
- the test substance was patched became spotty the measurement 6-10 mm with the distance not less than 3 cm.
- paper chromatography  was afterwards hung in the vessel by using the stick anti of siphon or clips held the tip of paper.
(beforehand was left first alone for a moment, to bored, in order to rise slowly or really was separated).
- was made an effort to get by paper that hanged down did not touch shelves or the wall of the vessel. The vessel was closed impermeable for saturation.

B.increase chromatography
the implement and the material were the same as descended chromatography , but the method : from top to bottom.